Coding
Part:BBa_K777109
Designed by: Flash Coli Group: iGEM12_Goettingen (2012-09-18)
fliC
- The fliC gene codes for flagellin. This protein is the subunit that forms the flagellar filament of E. coli.
Usage and Biology
We expected that transformation of E. coli with fliC constructs would results in longer flagella filaments. Unfortunately we were not able to confirm this via transmission electron microscopy. However we tested the transformed strains in swimming agar assays and they were significantly faster than control strains (Fig. 1). This was the case on M9 swimming agar and also on [http://2012.igem.org/Team:Goettingen/Project/Materials#Tryptone_swimming_agar tryptone swimming agar]. This indicates that fliC transformation indeed caused longer flagella filaments and thus higher motility in BL21 E. coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1224
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 301
Illegal AgeI site found at 709 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//function/motility
//function/motility
chassis | E. coli |
function | Motility |
protein | Flagellin |